av J Dunevall · 2018 — burst open due to the electric field at the polarized electrode and the vesicular cargo to form the aldehyde adduct, 3,4-dihydroxyphenylacetaldehyde (DOPAL). instructions in the form of DNA or RNA, which are then introduced to the cell by

Bifunctional electrophilic compounds such as HQ and p-BQ, acrolein, and VC metabolites, are all able to form exocyclic adducts . The platinum-based drug cisplatin is a widely used first-line therapy for several cancers. Cisplatin interacts with DNA mainly in the form of Pt-d(GpG) di-adduct, which stalls cell proliferation Based on these observations, it was concluded that pyridyloxobutyl DNA adducts formed at GC base pairs were mutagenic, at least in bacteria. However, we cannot rule out that adducts at AT base pairs are not mutagenic in this study since TA102 has an active NER system that could be repairing any mutagenic adducts at AT base pairs. Adducts were determined using HPLC/MS/MS by comparison to synthesized standards.

Dna adducts are formed due to

pyridyloxobutyl DNA adducts, a critical step in its mechanism of carcinogenesis. In addition to DNA nucleobase adducts, DNA phosphate adducts can be formed by pyridyloxobutyla-tion of the oxygen atoms of the internucleotidic phosphodiester linkages. We report the use of a liquid chromatography− nanoelectrospray ionization−high-resolution The levels of ellipticine-DNA adducts formed in rat tissues after their administration with ellipticine-micelles were one order of magnitude lower in most organs than in those of rats treated with free ellipticine (p < 0.001, different from levels of ellipticine-derived DNA adducts formed by free ellipticine; comparison was performed by t-test analysis), with an exception of brain, where are formed that directly bind with the DNA or may generate a cascade of electrophiles that further reacts or bind with DNA and produce DNA adducts (Figure 3). For volatile or acyclic nitrosamines, HCHO (formaldehyde) may acts as an alkylating agent and produce DNA adducts[34] (Figure 4).

The detection of the DNA adducts is of great DNA adduct formation in northern pike (Esox lucius) exposed to a mixture of benzo[a]pyrene, benzo[k]fluoranthene and 7H-dibenzo[c,g]carbazole: time-course DNA adduct formation appears to be a general response of plants to organic of DNA adducts, as well as the ways in which they can be eliminated due to DNA av S Johansson · 2009 · Citerat av 13 — The formation of these adducts most likely proceeds via the radical specific for the individual olefinic hydroperoxides due to the inclusion of a The ability of forming DNA adducts is compared through the formation enthalpy DNA adducts and may be used as prodrugs in a wide range of patients, due to Swedish University dissertations (essays) about DNA ADDUCTS.

2009-07-13

Adducts can be characterized by several criteria, including molecular size. Hence, the reference to large bulky adducts and small DNA adducts. Small DNA adducts can occur both from chemical exposure and from normal metabolic processes. Investigating DNA adduct formation by flavor chemicals and tobacco byproducts in electronic nicotine delivery system (ENDS) using in silico approaches Investigating DNA adduct formation by flavor chemicals and tobacco byproducts in electronic nicotine delivery system (ENDS) using in silico approaches M 1 G (pyrimido- [1,2- a ]purin-10 (3H)-one) adducts are formed in the reaction of DNA with MDA (malondialdehyde), the most abundant carbonyl product of lipid peroxidation [73].

Utvärdera styrkan av miljömässiga kemikalier och läkemedel, vara enzymatiskt bioaktiverade till intermediärer genererar kovalent DNA

Hallgard, Elna (författare); Modifying Cooking Practices to Reduce the Formation of Heterocyclic Amines; 2004; Doktorsavhandling (övrigt vetenskapligt)abstract. affecting agents, we do not know whether the effect is due to confounding. A positive hydrocarbon exposure, urinary mutagenicity, and DNA adducts in rubber Degradation Mechanism of Silver Metal Deposited on Lead Halide Perovskites Perovskites with Narrow Band Gap via Lewis Acid-Base Adduct Approach Direct Determination of Metal Complexes' Interaction with DNA by Atomic Telemetry Institutionaliseret etik har typisk form af universelle standarder, som. This is related to the medical industry and the growing presence of intense competition, In this study, we identified and characterized the disrupted gene in a T-DNA First Evidence for the Formation of a CO Cluster Adduct before CO Insertion.

COMPUTATIONAL STUDIES OF DAMAGED DNA: AN INVESTIGATION OF DNA O-LINKED ADDUCTS FORMED DUE TO EXPOSURE TO PHENOLIC CARCINOGENS MOHADESEH MAJDI YAZDI B.Sc., Sharif University of Technology, 2004 M.Sc., Tarbiat Modares University, 2008 A Thesis Submitted to the School of Graduate Studies of the University of Lethbridge in Partial Fulfillment of the Analysis of DNA adducts formed in vivo can help to improve risk assessment and contribute to better understanding of the mechanisms of chemical carcinogenesis. The main parts of the thesis are characterisation of DNA adducts formed by the studied epoxides, development of quantitative methods for the analysis of epoxideinduced adducts and analysis of such adducts in animal and human tissue … 17 hours ago Due to its low toxicity, DMPO can be used in cells at high enough concentrations to out-compete the normal reactions of DNA radicals, thus ensuring a high yield of DNA nitrone adducts. Because A structurally-related adduct of sterigmatocystin formed a similar intercalated structure (47). Unlike most DNA adducts, the AFB 1 N7-dG adducts thermodynamically stabilized the DNA duplex, as evidenced by increases in duplex melting temperatures (T m studies), as monitored either by … DMBA-DNA adducts formed in mouse epidermis by enantiomers of tram-3,4-dihydrodiol via their DMBA-3,Cdihydrodiol 1,2-epoxides have been reported (8).
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DNA adduct formation is dependent on 1) exposure to, absorption of, retention of and distribution of toxic substances and, 2) nutritional status, which in turn inﬂuences toxin absorption, retention, etc, In addition, new evidence is provided that DNA adducts are formed in target mammary cells (4.7-fold higher than in controls). Although DNA adducts do not necessarily evolve into tumours or other chronic degenerative diseases, the formation of these molecular lesions in target mammary cells may bear relevance for the potential involvement of BPA in breast carcinogenesis. interaction of carcinogens with DNA. DNA adducts are typically formed in very low concentrations in vivo «100 fmol/pg DNA; <3 adducts/105 nucleotides), which has made their detection difficult; however, with the advent of immunoassays (Poirier, 1984) and 32P-postlabelling (Gupta et al., 1982), their occurrence in exposed human populations has become The two DNA adducts of benzo[a]pyrene (BP) previously identified in vitro and in vivo are the stable adduct formed by reaction of the bay-region diol epoxide of BP (BPDE) at C-10 with the 2-amino These adducts stabilised DNA such that they functioned as virtual interstrand crosslinks. The interstrand crosslinks were formed in the presence of mitoxantrone and formaldehyde in a time- and concentration-dependent manner.

The results link gene research with the nutritional and environmental approaches to medicine. Early indications are that the burden of DNA adducts may be reduced by nutritional intervention. Mutations in critical genes can result in malignant transformation. Several techniques are available with sufficient sensitivity to detect DNA adducts formed as a consequence of occupational or environmental exposure to carcinogens.
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Investigating DNA adduct formation by flavor chemicals and tobacco byproducts in electronic nicotine delivery system (ENDS) using in silico approaches Investigating DNA adduct formation by flavor chemicals and tobacco byproducts in electronic nicotine delivery system (ENDS) using in silico approaches

We have isolated and purified this adduct formed under oxido-reductive (Fenton) conditions in Tris buffer. We show by homo- and heteronuclear NMR spectroscopy that the covalent Adriamycin-DNA adduct is structurally We are involved in a systematic search for novel mutagenic lesions formed by oxidation.

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DNA adducts in many human tissues [12]. In 2012, Phillips updated this topic and reviewed studies on tobacco smoke-related DNA adducts published since 2002 [10]. That review covered the chemical nature and origins of smoking-related DNA adducts and the effect of DNA repair and gene polymorphisms on the levels of DNA adducts in humans.

Therefore, an analytical method aimed at detecting DNA adducts … Chemicals that form DNA adducts include: acetaldehyde, a significant constituent of tobacco smoke cisplatin, which binds to DNA and causes crosslinking, leading to death of the cell DMBA ( 7,12-dimethylbenz (a)anthracene) malondialdehyde, a naturally occurring product of lipid peroxidation DNA adducts are covalent interactions between reactive carcinogen chemical species and DNA (usually genomic). Adducts can be characterized by several criteria, including molecular size.